im sure some of you ill-informed homebrewers will flame me for not baking, so please read little grasshoppers......
Why I don't autoclave/bake my gear. - By SV-1
I've seen a lot of info on the board lately about how using an autoclave or pressure cooker will sterilize oil based AAS, well after a good bit of research everything I've been able to find states the exact opposite.
To start here is a quote by Justin, a moderator at UK Muscle:
Quote:
Clearing up some misconceptions
Heat sterilisation of oil will not be successful by using 250F.
250F is the heat used by a certain class of autoclave. The autoclave uses pressure also to achieve wet sterilisation, this method will work for aqueous solutions but will NOT sterilise oil. If heat is the chosen method to sterilise oil, then you must sterilise by dry heat methods, i.e. 150-170C (302 - 338F) for 1-4 hrs, (type and volume depending) which can be detrimental to certain hormone preparations.
Divert your attentions to using clean practises, filtering with a 0.22um membrane filter and incorporating a Bacteriostatic agent (BA).
I found the following to support this quote.
From the FLINDERS UNIVERSITY OF SOUTH AUSTRALIA - FACULTY OF SCIENCE AND ENGINEERING
SCHOOL OF BIOLOGICAL SCIENCES USE AND TRAINING FOR AUTOCLAVES
"Ensure that the material is autoclavable – Oils, waxes, some plastics, flammable materials and samples
containing solvents or substances that may emit toxic fumes should not be autoclaved"
Finally here is an advertisement for an ALL AMERICAN Electric Autoclave Model 25X, a $750 Sterilizer/Autoclave. Which says very clearly in the last line, "Not to be used to sterilize oils and powders."
So if you're counting on autoclaving/pressure cooking or baking (at any temp/time that wont fry your gear) to give you a sterile oil based product, think again.
those are just little bits and pieces i threw together, for full article with plenty of links PM me