My experience is once it’s mixed regardless of it’s a 2mg vial or a 5mg vial am effective dose would cause you to run through the bottle in either 2 or 5 days max. I’ve heard people talking about 14 days after it’s been reconstituted it begins to break down. But that’s hearsay. I’ve spent weeks reading up on it and haven’t found anything concrete. I assume since it’s recommended at a dose of 1mg per day people assume it’s gone in a few days so it’s shelf life shouldn’t matter.