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igf for dummies

SouthernMuscle

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about to order some igf from one of our fine sponsors here. the kit comes with 1mg igf powder, 2ml acetic acid, and 10ml NaCL

now can someone please explain to me the protocol step by step for using/storing/mixing this?
 
for God's sake I know you people use this stuff! Quit holding out on me:D

I've got the jist of it, just not the freezing and reconstituting
 
Thought I'd bump this up too.

I saw this kit and not sure what the NaCL is for either.

SoutherMuscle..

MikeS posted a "how to" a while back when he did one of the Gensci kits - you might get some basic info there. Still not sure what the NaCL is for though.
 
--Put 2 ml aa into the vial. Point the needle towards the side of the vial, do not let it hit the powder directly. The vial is vacuum sealed. It will pull the plunger as soon as you insert the needle. I use a slin rig for this, others use a 3ml. The 3ml is less accurate. So either hold the plunger to slow the rate of the aa going into the vial, or if you are using a smaller gauge slin pin I just point the needle to the side of the vial and let the vacuum pull the aa in.

--Now you have 500mcg/ml concentration. Do not shake. Swirl gently. The powder will go into solution by itself, but I swirl in an attempt to free up any IGF that may be trapped in the grooves of the glass. You can't see this with the naked eye of course so don't bother looking, lol.

--Every .02ml is 10mcg of IGF.

--Pull up about .2-.3ml NaCl. Pull up your IGF. Be careful not to introduce NaCl into the IGF vial. Done. Some pull up their IGF 1st. I do it 2nd so its at the end of the rig and gets a good flush so there is no IGF left in the rig.

Got it?

Rex.
 
excellent explanation, could not have been clearer

there's still one thing I don't get though, I've heard of freezing the portion you're not using, so would one just use the protocol you outlined and store the vial in the freezer?

thanks
 
SouthernMuscle said:
excellent explanation, could not have been clearer

there's still one thing I don't get though, I've heard of freezing the portion you're not using, so would one just use the protocol you outlined and store the vial in the freezer?

thanks

No need to freeze. Its good for up to 2 yrs in the fridge in aa.

Rex.
 
first remove the vacuum from the vial, by injecting air into it. when the vacuum is gone it will no longer pull the plunger down

you can use as much AA as you like. the original kits came with 1ml. most time when i drew it into a syringe, it was't even that much, which of course is a problem. chemone includes a little more than 2ml so if you want to use 1ml, fine, 2ml, fine. there will be enough.

i personally use 2ml because it is easier to measure later when you draw it into a syring.

after removing the vacuum SLOWLY add AA to the vial. try not to let the stream of aa hit the powder directly, try to let it run down the glass into the powder. when the aa is in gently swirl the vial until all is disolved. do not shake.

a 1ml syringe is marked like this llll10llll20llll30llll40llll50llll60...etc
a 1/2 ml syringe is like this llll5llll10llll15llll20llll25llll30

on a 1ml each line represents 2 units 2,4,6,8,10
on a 1/2 ml each line is 1 unit 1,2,3,4,5,6,7,8,9,10. this makes it easier to measure.

so if you use 1ml of aa, on a 1ml pin, each line is 20mcg. on a 1/2ml pin each line is 10mcg

if you use 2ml of aa, on a 1ml pin each line is 10mcg, on a 1/2 ml each line is 5mcg...the 5line is 25mcg, 10 line is 50mcg...etc

so you see that the easiest way to measure is using 2ml and a 1/2ml pin

your igf is stored in aa solution, nacl is added to dilute at the time of injection. draw twice as much nacl as igf. so if you draw igf to the ten line, draw nacl to the 30 line and you are ready to go.

normally i do this by PM where i can answer individual questions. in a post i tried to cover every possibility, making this the most complete and possibly most confusing version yet....sorry about that.

feel free to post your questions, i probably deserve it...lol

chemone's kits are exactly how they should be. a true .6% aa solution and nacl(saline solution) to dilute. diluting lessens the sting from the acid and prevents tissue necrosis.

C
 
the igf i have didn't come with dilutents...so i had them and did it myself,the aa burns like hell,i diluted it a bit more and still kills me.
what can i do to lessen this sting,it is almost unbearable.
it's sitting in the fridge waiting to kill me:)
 
ryjenn said:
the igf i have didn't come with dilutents...so i had them and did it myself,the aa burns like hell,i diluted it a bit more and still kills me.
what can i do to lessen this sting,it is almost unbearable.
it's sitting in the fridge waiting to kill me:)
chances are that the AA is much too strong. some sources give a 5% solution, when it should be 6/10%...a .6% solution is the perfect PH environment for the peptide.

just draw your igf and then fill the syringe with nacl or and sterile water. it's the best you can do.

to make your own aa, get distilled white vinigar, dilute 8/1 with sterile water. this is very close to a perfect .6% aa solution.
 
chaos1 said:
chances are that the AA is much too strong. some sources give a 5% solution, when it should be 6/10%...a .6% solution is the perfect PH environment for the peptide.

just draw your igf and then fill the syringe with nacl or and sterile water. it's the best you can do.

to make your own aa, get distilled white vinigar, dilute 8/1 with sterile water. this is very close to a perfect .6% aa solution.


thanks chaos...i purchased the aa from a chemical supplier and i did think it was possibly too strong,did that kill the igf???
i won't even bother using it if that is the case..
i added twocc of aa and then added 1cc of bw just to dilute if further,still really burns.
if i keep diluting it how am i measuring the doses????
if i add another ml of bw i will be at 4ml of dilutent to 1mg of igf
 
ryjenn said:
thanks chaos...i purchased the aa from a chemical supplier and i did think it was possibly too strong,did that kill the igf???
i won't even bother using it if that is the case..
i added twocc of aa and then added 1cc of bw just to dilute if further,still really burns.
if i keep diluting it how am i measuring the doses????
if i add another ml of bw i will be at 4ml of dilutent to 1mg of igf
i cant say for sure, but worst case is it's degrading, and not as strong as it once was. just use it in a hurry, and it SHOULD be fine.

do you know the % of AA you bought? i know that researchlabsupply sells it prediluted to the right strength.

with 4ml of dilutent, each unit is 2.5mcg. so the 5 line is 12.5mcg, the 20 line is 50mcg, and so on.

another thing to remember. bac water is not the best choice because it contains .9% BA. it's a stronger acid solution than you are supposed to store in. sterile water, or filtered distilled water are better.
 

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