According to Novozymes who holds the patent on Long R3 IGF-1, if you reconstitute with AA it is good for several months. If by bac water maybe 20 to 30 days. There is 2000mcg in a vial of our Long R3 IGF-1, so that is 20 days if your in vitro research calls for 100mcg per day. Now if you don't want to risk the product degrading you can separate the reconstituted Long R3 IGF-1 in aliquots of 100mcg each in individual syringes and freeze them until the in vitro research is ready to commence.