Klotho Protein Peptide

[whacked]

This is more of a multifactorial, anti-aging peptide (think Epitalon, SS-31, etc) but more impressive (on paper).

I’m seeing this being discussed on several platforms and a few RC sites claim they will be offering it very soon.

We are not supposed to post Google or AI info so here are some cliff notes:

-Improved Longevity via promotion of youthful cells and tissues.
-Increased Life Span / biological age reversal
-Kidney health
-Cardiovascular protection
-Skin Health
-Bone health
-Neuro-protective, brain health and cognitive enhancement (being studied as an Alzheimer’s treatment and preventive)
-Reduces inflammation and oxidative stress
-Protects blood vessels
-Promotes autophagy
-Prevents sarcopenia

 

[buck]

A quick search( as i am always looking to be younger if i can live forever) shows possible skeletal and kidney issues. Wonder how often they pop up with it's use.

 

[whacked]

A quick search( as i am always looking to be younger if i can live forever) shows possible skeletal and kidney issues. Wonder how often they pop up with it's use.

Same here man!

I’m not reading issues but that rather benefits for the kidneys (restoring function and overall health).

Ditto as it relates to “skeletal issues” as it relates to bone health and sarcopenia prevention.

 

[Stewie]

From my understanding it's not a simple mix-and- go protein to synthesize. It's a large very globular protein to synthesize at 1012 aa long. Fairly long chain that requires some very expensive equipment and mammal cell cultures. Nor is it very stable. There's shorter peptides that are a bit easier to make. Although, in comparison to full-lenght klotho, not close in comparison. Lacks several post translation gene expressions.

 

[Stewie]

From my understanding it's not a simple mix-and- go protein to synthesize. It's a large very globular protein to synthesize at 1012 aa long. Fairly long chain that requires some very expensive equipment and -mammalian- cell cultures. Nor is it very stable. There's shorter peptides that are a bit easier to make. Although, in comparison to full-lenght klotho, not close in comparison. Lacks several post translation gene -modifications-.

Late—late corrections–emend -hyphen-.

 

[Stewie]

.

 

[whacked]

Same here man!

I’m not reading issues but that rather benefits for the kidneys (restoring function and overall health).

Ditto as it relates to “skeletal issues” as it relates to bone health and sarcopenia prevention.

Snippet on restoring Kidney health via addressing Klotho regression as we age (thus increasing, reversal; albeit I took the liberty to connect some dots as this is not directly cited here but is elsewhere).

The Renal Pacemaker: How Kidney Senescence Drives Systemic Aging

Longevity research has long treated the kidney as a passive victim of aging, merely a filter that clogs over time. A comprehensive new review challenges this view, positing that the kidney acts as a central “pacemaker” for systemic aging. The mechanism is a vicious feedback loop between cellular...

www.rapamycin.news

Klotho Protein Restores Regenerative Capacity in Aging Tissues | Herna De Wit, PhD Biochemistry, LLB Law posted on the topic | LinkedIn

Longevity isnât just about slowing decline. Itâs about restoring regeneration. A new npj Aging (Nature partner) paper just gave us a glimpse of what that could look like. Researchers supplemented ageing mice with soluble α-Klotho, a protein often called an anti-ageing hormone. The results...

www.linkedin.com

Klotho Supplementation Reverses Renal Dysfunction and Interstitial Fibrosis in Remnant Kidney​

Abstract​

Introduction: While recent investigations show that klotho exerts renoprotective actions, it has not been fully addressed whether klotho protein supplementation reverses renal damage. Methods: The impacts of subcutaneous klotho supplementation on rats with subtotal nephrectomy were examined. Animals were divided into 3 groups: group 1 (short remnant [SR]): remnant kidney for 4 weeks, group 2 (long remnant [LR]): remnant kidney for 12 weeks, and group 3 (klotho supplementation [KL]): klotho protein (20 μg/kg/day) supplementation on the remnant kidney. Blood pressure, blood and urine compositions with conventional methods such as enzyme-linked immunosorbent assay and radioimmunoassay, kidney histology, and renal expressions of various genes were analyzed. In vitro studies were also performed to support in vivo findings. Results: Klotho protein supplementation decreased albuminuria (−43%), systolic blood pressure (−16%), fibroblast growth factor (FGF) 23 (−51%) and serum phosphate levels (−19%), renal angiotensin II concentration (−43%), fibrosis index (−70%), renal expressions of collagen I (−55%), and transforming growth factor β (−59%) (p < 0.05 for all). Klotho supplementation enhanced fractional excretion of phosphate (+45%), glomerular filtration rate (+76%), renal expressions of klotho (+148%), superoxide dismutase (+124%), and bone morphogenetic protein (BMP) 7 (+174%) (p < 0.05 for all). Conclusion: Our data indicated that klotho protein supplementation inactivated renal renin-angiotensin system, reducing blood pressure and albuminuria in remnant kidney. Furthermore, exogenous klotho protein supplementation elevated endogenous klotho expression to increase phosphate excretion with resultant reductions in FGF23 and serum phosphate. Finally, klotho supplementation reversed renal dysfunction and fibrosis in association with improved BMP7 in remnant kidney.

 

[Stewie]

Snippet on restoring Kidney health via addressing Klotho regression as we age (thus increasing, reversal; albeit I took the liberty to connect some dots as this is not directly cited here but is elsewhere).

The Renal Pacemaker: How Kidney Senescence Drives Systemic Aging

Longevity research has long treated the kidney as a passive victim of aging, merely a filter that clogs over time. A comprehensive new review challenges this view, positing that the kidney acts as a central “pacemaker” for systemic aging. The mechanism is a vicious feedback loop between cellular...

www.rapamycin.news

Klotho Protein Restores Regenerative Capacity in Aging Tissues | Herna De Wit, PhD Biochemistry, LLB Law posted on the topic | LinkedIn

Longevity isnât just about slowing decline. Itâs about restoring regeneration. A new npj Aging (Nature partner) paper just gave us a glimpse of what that could look like. Researchers supplemented ageing mice with soluble α-Klotho, a protein often called an anti-ageing hormone. The results...

www.linkedin.com

Klotho Supplementation Reverses Renal Dysfunction and Interstitial Fibrosis in Remnant Kidney​

Abstract​

Introduction: While recent investigations show that klotho exerts renoprotective actions, it has not been fully addressed whether klotho protein supplementation reverses renal damage. Methods: The impacts of subcutaneous klotho supplementation on rats with subtotal nephrectomy were examined. Animals were divided into 3 groups: group 1 (short remnant [SR]): remnant kidney for 4 weeks, group 2 (long remnant [LR]): remnant kidney for 12 weeks, and group 3 (klotho supplementation [KL]): klotho protein (20 μg/kg/day) supplementation on the remnant kidney. Blood pressure, blood and urine compositions with conventional methods such as enzyme-linked immunosorbent assay and radioimmunoassay, kidney histology, and renal expressions of various genes were analyzed. In vitro studies were also performed to support in vivo findings. Results: Klotho protein supplementation decreased albuminuria (−43%), systolic blood pressure (−16%), fibroblast growth factor (FGF) 23 (−51%) and serum phosphate levels (−19%), renal angiotensin II concentration (−43%), fibrosis index (−70%), renal expressions of collagen I (−55%), and transforming growth factor β (−59%) (p < 0.05 for all). Klotho supplementation enhanced fractional excretion of phosphate (+45%), glomerular filtration rate (+76%), renal expressions of klotho (+148%), superoxide dismutase (+124%), and bone morphogenetic protein (BMP) 7 (+174%) (p < 0.05 for all). Conclusion: Our data indicated that klotho protein supplementation inactivated renal renin-angiotensin system, reducing blood pressure and albuminuria in remnant kidney. Furthermore, exogenous klotho protein supplementation elevated endogenous klotho expression to increase phosphate excretion with resultant reductions in FGF23 and serum phosphate. Finally, klotho supplementation reversed renal dysfunction and fibrosis in association with improved BMP7 in remnant kidney.

So you're aware, the klotho fragmented truncated protein fragment they used in the citation you cited is still a pretty big aa sequence at 516aa long. Much bigger and more complex to synthesize than any underground research company will mass produce.

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www.thermofisher.com

$225 per 20ug is a modest price used for these murine models. Take that and multiply it to a HED– which there's no established clinical HED.

If you really want to try and uptick klotho expression. Look into SIRT1, your vit D status, ACE inhibition (part of a reason why I take lisinopril) and and histone deacetylation. There's other modulators.

Caveat emptor my man.

 

[whacked]

So you're aware, the klotho fragmented truncated protein fragment they used in the citation you cited is still a pretty big aa sequence at 516aa long. Much bigger and more complex to synthesize than any underground research company will mass produce.

Loading…

www.thermofisher.com

$225 per 20ug is a modest price used for these murine models. Take that and multiply it to a HED– which there's no established clinical HED.

If you really want to try and uptick klotho expression. Look into SIRT1, your vit D status, ACE inhibition (part of a reason why I take lisinopril) and and histone deacetylation. There's other modulators.

Caveat emptor my man.

Very cool
Thanks Stewie
I always appreciate your contributions and input!