New 2016 study - Thymosin Beta-4/ TB-500... Good for teeth

[johnjuanb1]

The periodontal ligament, commonly abbreviated as the PDL, is a group of specialized connective tissue fibers that essentially attach a tooth to the alveolar bone within which it sits.

Thymosin Beta-4 Suppresses Osteoclastic Differentiation and Inflammatory Responses in Human Periodontal Ligament Cells.
Lee SI, et al. PLoS One. 2016.

Abstract

BACKGROUND: Recent reports suggest that thymosin beta-4 (Tβ4) is a key regulator for wound healing and anti-inflammation. However, the role of Tβ4 in osteoclast differentiation remains unclear.

PURPOSE: The purpose of this study was to evaluate Tβ4 expression in H2O2-stimulated human periodontal ligament cells (PDLCs), the effects of Tβ4 activation on inflammatory response in PDLCs and osteoclastic differentiation in mouse bone marrow-derived macrophages (BMMs), and identify the underlying mechanism.

METHODS: Reverse transcription-polymerase chain reactions and Western blot analyses were used to measure mRNA and protein levels, respectively. Osteoclastic differentiation was assessed in mouse bone marrow-derived macrophages (BMMs) using conditioned medium (CM) from H2O2-treated PDLCs.

RESULTS: Tβ4 was down-regulated in H2O2-exposed PDLCs in dose- and time-dependent manners. Tβ4 activation with a Tβ4 peptide attenuated the H2O2-induced production of NO and PGE2 and up-regulated iNOS, COX-2, and osteoclastogenic cytokines (TNF-α, IL-1β, IL-6, IL-8, and IL-17) as well as reversed the effect on RANKL and OPG in PDLCs. Tβ4 peptide inhibited the effects of H2O2 on the activation of ERK and JNK MAPK, and NF-κB in PDLCs. Furthermore, Tβ4 peptide inhibited osteoclast differentiation, osteoclast-specific gene expression, and p38, ERK, and JNK phosphorylation and NF-κB activation in RANKL-stimulated BMMs. In addition, H2O2 up-regulated Wnt5a and its cell surface receptors, Frizzled and Ror2 in PDLCs. Wnt5a inhibition by Wnt5a siRNA enhanced the effects of Tβ4 on H2O2-mediated induction of pro-inflammatory cytokines and osteoclastogenic cytokines as well as helping osteoclastic differentiation whereas Wnt5a activation by Wnt5a peptide reversed it.

CONCLUSION: In conclusion, this study demonstrated, for the first time, that Tβ4 was down-regulated in ROS-stimulated PDLCs as well as Tβ4 activation exhibited anti-inflammatory effects and anti-osteoclastogenesis in vitro. Thus, Tβ4 activation might be a therapeutic target for inflammatory osteolytic disease, such as periodontitis.

 

[TODouble2]

i dont know what most of that study means, but i remembered you posted this study and noticed something....

i had really bad tooth pain in one of my teeth for weeks.. a sharp chilling pain that would come often. people said i probably need root canal. all of a sudden it went away and i read this post a few weeks ago.

it coincidentally went away right around the time i started taking tb500.

i ran out of TB500 about two weeks ago, and i just had the pain come back..

very strange. it definietly made wonder.

 

[johnjuanb1]

i dont know what most of that study means, but i remembered you posted this study and noticed something....

i had really bad tooth pain in one of my teeth for weeks.. a sharp chilling pain that would come often. people said i probably need root canal. all of a sudden it went away and i read this post a few weeks ago.

it coincidentally went away right around the time i started taking tb500.

i ran out of TB500 about two weeks ago, and i just had the pain come back..

very strange. it definietly made wonder.

That is interesting. I need to order more BPC157. I might as well get TB-500 also. My body fat is low and my old injuries feel unsafe while lifting lately.