Buy Needles And Syringes With No Prescription
M4B Store Banner
ddd
Riptropin Store banner
Generation X Bodybuilding Forum
Buy Needles And Syringes With No Prescription
Buy Needles And Syringes With No Prescription
Mysupps Store Banner
IP Gear Store Banner
Anabolic Hormones Store Banner
Ganabol Store Banner
Spend $100 and get bonus needles free at sterile syringes
Professional Muscle Store open now
LandmarkChem Email Banner
Medtech Store Banner
Bruce Labs Store banner
qtropin
Professional Muscle Store open now
over 5000 supplements on sale at professional muscle store
Buy Needles And Syringes With No Prescription
ESPECIL-2
Buy Needles And Syringes With No Prescription
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store
over 5000 supplements on sale at professional muscle store

New muscle specific peptide? Anabolic?

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
I don't know if this has been covered here but I found this research interesting! Here is a cut and paste quote for you peptide freaks! Check out the link. Is this a pep that our resident peptide companies could get their hands on? Phil?

Small peptide for muscle regeneration-University of Santiago de Compostela

"Obestatin, a 23-amino acid peptide encoded by the ghrelin gene, and the GPR39 receptor are expressed in rat skeletal muscle and are up-regulated upon experimental injury.
Obestatin promotes migration, proliferation and differentiation of myoblasts, three processes that are essential for muscle regeneration. Its administration promoted the exit of the cell cycle, the expression of myogenic transcription factors, the migration of myoblasts and the fusion of myoblasts in order to determine the myotubes. In vitro assays showed that obestatin:
• Promotes myogenesis through the expression of myogenin and MHC (Myosin Skeletal Heavy Chain) on a dosis-dependent manner in rat L6E9 myoblasts.
• Acivates the main mitogenic and myogenic factors (Akt, p38, pERK) in both L6E9 myoblasts and myotubes.
• Induces an increase in the cell number of myoblasts at 48h after stimulation.
• Promotes differentiation of myoblasts (Increases 2.5 times the differentiation index respect to control) as well as the fusion of myoblasts in order to determine the myotubes (increase in the fusion index: 20% (control) versus 70% (+obestatin) of myotubes with >4 nucleus)"

This means it will have a regenerative effect on muscle tissue at the least if not a anabolic one... Or am I dumb? Lol
 

johnjuanb1

Featured Member / Sponsor Rep
Featured Member
Kilo Klub Member
Registered
Sponsors
Joined
Mar 6, 2009
Messages
25,593
If you can get the amino acid sequence, Phil Hernon can have it synthesized in his new USA LAB for ergopep. Sounds promising!
 

paloboi

New member
Registered
Joined
May 10, 2011
Messages
204
looks pretty promising and has the added plus of being easy to synth in theory but we know how muscle mice studies usually turn out for us :D
 
Last edited:

workhard2121

Active member
Kilo Klub Member
Registered
Joined
Sep 6, 2010
Messages
1,710
Looks very interesting. Im sure Phil can have this done if worth it. JJ is right on the money!
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
looks pretty promising and has the added plus of being easy to synth in theory but we know how muscle mice studies usually turn out for us :D
True but how many things are we willing to try when it's only been shown to be anabolic in a Petrie dish?!? At least this has had some animal studies. Just think of recovering in one day instead of 2-3... How much of a difference could that make? Specially for the guys getting up there in age. Plus being so specific to muscle cells it would be easy to stack with things like gh or aas which have a more systematic effect and more side effects. Assuming that it has a very low side effect profile as most peptides do. It could also be utilized post cycle to maintain the enhanced recovery that aas normaly provides. it could possibly be used coming into a stringently tested athletic event without fear of testing positive to any banned substances. Just a few thoughts...
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Found this also. Seems it regenerated heart tissue and function...

Obestatin induced recovery of myocardial dysfunction in type 1 diabetic rats: underlying mechanisms.

AuthorsAragno M, et al. Show all Journal
Cardiovasc Diabetol. 2012 Oct 15;11:129. doi: 10.1186/1475-2840-11-129.

Affiliation
Department of Experimental Medicine and Oncology, University of Turin, Corso Raffaello 30, Turin, 10125, Italy. [email protected]

Abstract
BACKGROUND: The aim of this study was to investigate whether obestatin (OB), a peptide mediator encoded by the ghrelin gene exerting a protective effect in ischemic reperfused heart, is able to reduce cardiac dysfunctions in adult diabetic rats.

METHODS: Diabetes was induced by STZ injection (50 mg/kg) in Wistar rats (DM). OB was administered (25 μg/kg) twice a day for 6 weeks. Non-diabetic (ND) rats and DM rats were distributed into four groups: untreated ND, OB-treated ND, untreated DM, OB-treated DM. Cardiac contractility and ß-adrenergic response were studied on isolated papillary muscles. Phosphorylation of AMPK, Akt, ERK1/2 and GSK3ß as well ß-1 adrenoreceptors levels were detected by western blot, while α-MHC was measured by RT-PCR.

RESULTS: OB preserved papillary muscle contractility (85 vs 27% of ND), ß-adrenergic response (103 vs 65% of ND), as well ß1-adrenoreceptors and α-MHC levels in diabetic myocardial tissue. Moreover, OB up-regulated the survival kinases Akt and ERK1/2, and enhanced AMPK and GSK3ß phosphorylation. OB corrected oxidative unbalance, reduced pro-inflammatory cytokine TNF-α plasma levels, NFkB translocation and pro-fibrogenic factors expression in diabetic myocardium.

CONCLUSIONS: OB displays a significant beneficial effect against the alterations of contractility and ß-adrenergic response in the heart of STZ-treated diabetic rats, which was mainly associated with the ability of OB to up-regulate the transcription of ß1-adrenergic receptors and α-MHC; this protective effect was accompanied by the ability to restore oxidative balance and to promote phosphorylation/modulation of AMPK and pro-survival kinases such as Akt, ERK1/2 and GSK3ß.

Obestatin induced recovery of myocardia - PubMed Mobile
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Another positive that we see with a lot of peptides... This reasearch indicates that Obestatin is a potent vaso relaxer via no activation! In bodybuilding terms it facilitates increased blood flow to the extremities (pumps!).

The gastrointestinal peptide obestatin induces vascular relaxation via specific activation of endothelium-dependent NO signalling.

The gastrointestinal peptide obestatin - PubMed Mobile
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
It keeps getting better!

Obestatin induces testosterone secretion from rat testis
in vitro

Hizbullah, Sarwat Jahan* and Shakeel Ahmed
Reproductive physiology lab, Department of Animal sciences Quaid-i-Azam University, Islamabad. Pakistan.
Accepted 10 March, 2011
In this study, the effect of obestatin (23 amino acid peptide) on testosterone secretion in vitro, in the rat testis was observed. For this purpose, two different doses of obestatin (10-9 M and 10-8 M) were used alone and in combination with human chorionic gonadotropin (hCG) in fasting and fed conditions in two age groups. Fasting induced a significant reduction in body weight (p < 0.05) and plasma testosterone concentrations (0.001). hCG stimulated testosterone secretions were significantly (p < 0.05) high as compared to the basal control testosterone concentrations after 90 min in some groups and 180 min of incubation in all groups. Obestatin at the dose of 10-9 M alone and in combination with hCG failed to change testosterone concentrations in all groups; however, 10-8 M obestatin significantly (p < 0.05) induced hCG stimulated testosterone concentrations in both normally fed pre-pubertal and adult rats. No significant difference was noticed in 48 h fasted groups. This data suggests that, obestatin is a positive modulator of testosterone secretion and its effect depends upon the nutritional status of the body.

Full article: http://www.ajol.info/index.php/ajb/article/download/93551/82976
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
I don't know what to take from this paper but someone with more in depth research analysis skill may glean some useful information from it.

Effects of Circuit Resistance Training Intensity on the Plasma Ghrelin to Obestatin Ratios in Healthy Young Women: http://endometabol.com/7719.pdf
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
If you can get the amino acid sequence, Phil Hernon can have it synthesized in his new USA LAB for ergopep. Sounds promising!
I believe the letter sequence below each obestatin type is representative of it exact amino acid chain. Am I correct in this assumption?
 

Attachments

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
So from my reading it looks like Obestatin was initially reported to be involved in decreased food intake makeing it useful as a anti obesity compound... these findings have been refuted by a few recent studies but not clearly disproved. Recent research show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, positively affecting cell migration, proliferation and differentiation, increasing insulin sensitivity, improving testicular function, fighting fatty liver, being a vaso relaxant and protector, and increasing the secretion of pancreatic enzymes. Who knows what other positive benefits Obestatin may posses. To me I certainly seems it could be utilized by the bodybuilding and athletic cummunities in a number of interesting ways. Namely PCT. It seems to rejuvenate many tissues besides muscle! Heart, liver, testis, pancreas, and the vascular system! That's pretty fuckin cool
In my view. I don't think some athletes really realize how much stress we put out bodies through to achieve the extreme results we desire. Anything that can further our progression toward these goals and at the same time fight the bodily damage that come with that progress is a major find!
 

Cerberus777

Active member
Kilo Klub Member
Registered
Joined
May 14, 2008
Messages
3,417
Ive been trying to read up on this in my limited spare time. It seams to not only signal muscle building but any damaged tissue. Joints, tendons, organs...also helps with lowering blood pressure and increasing insulin sensitivity. Almost sounds to good to be true.

Phil. If you can get this made. It SHOULD be a win-win for you and any one researching it.
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Ive been trying to read up on this in my limited spare time. It seams to not only signal muscle building but any damaged tissue. Joints, tendons, organs...also helps with lowering blood pressure and increasing insulin sensitivity. Almost sounds to good to be true.

Phil. If you can get this made. It SHOULD be a win-win for you and any one researching it.
Also read some research on a peg stabilized version which lowered triglycerides 40%! Do you have any links you could share concerning joints and tendons?!?
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Unacylated ghrelin and obestatin increase islet cell mass and prevent diabetes in streptozotocin-treated newborn rats.

Abstract
The ghrelin gene products, namely acylated ghrelin (AG), unacylated ghrelin (UAG), and obestatin (Ob), were shown to prevent pancreatic b-cell death and to improve b-cell function under treatment with cytokines, which are major cause of b-cell destruction in diabetes. Moreover, AG had been described previously to prevent streptozotocin (STZ)-induced diabetes in rats; however, the effect of either UAG or Ob has never been examined in this context. In the present study, we investigated the potential of UAG and Ob to increase islet b-cell mass and to reduce diabetes at adult age in STZ-treated neonatal rats. One-day-old rats were injected with STZ and subsequently administered with either AG, UAG or Ob for 7 days. On day 70, plasma glucose levels, plasma and pancreatic insulin levels, pancreatic islet area and number, insulin and pancreatic/duodenal homeobox-1 (Pdx1) gene expression, and antiapoptotic BCL2 protein expression were determined. Similarly to AG, both UAG and Ob counteracted STZ-induced high glucose levels and improved plasma and pancreatic insulin levels, which were reduced by the diabetogenic compound. UAG and Ob increased islet area, islet number, and b-cell mass with respect to STZ treatment alone. Finally, in STZ-treated animals, UAG and Ob up-regulated insulin and Pdx1 mRNA and increased the expression of BCL2 similarly to AG. Taken together, our results suggest that in STZ-treated newborn rats, UAG and Ob improve glucose metabolism and preserve islet cell mass, granting a therapeutic potential in medical conditions associated with impaired b-cell function.
Journal of Molecular Endocrinology (2010) 45, 9–17

Full article: http://jme.endocrinology-journals.org/content/45/1/9.full.pdf
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Possible Modulation of Testosterone Secretion by Obestatin in Pubertal Male Rats
Sarwat Jahan*, Shakeel Ahmed, Nazifa Taqvim and Hizbullah
Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-AzamUniversity, Islamabad.

Abstract.- The objective of the present study was to examine the effect of different doses of obestatin on testosterone secretion in pubertal male Sprague Dawley rats. Animals were divided into three groups. One group served as control and the other two groups were treated with 8 and 16 nmol/kg of obestatin, respectively. Prior to sample collection, teflon cannula was implanted in lateral tail vein of rat and the sequential blood samples were collected 10 min before, at the time of obestatin treatment (0 min) and then at 10 min intervals upto 40 min. in heparinized syringes. The whole sampling was carried out under diethyl ether anesthesia. Plasma testosterone levels were determined by using Enzyme Immunoassay (EIA). Administration of obestatin (8 nmol/kg) in animals resulted in a significant (p<0.05) increase in mean plasma testosterone concentrations at 10 and 20 min after obestatin administration. Highly significant (p<0.001) increase in testosterone concentrations were observed at 30 min which then declined at 40 min. At dose of 16 nmol/kg in pubertal animals, a significant increase (p<0.05) was noticed in testosterone secretion at 20 and at 30 min after obestatin administration. Plasma testosterone concentrations then declined after 40 min. The present study suggests that this peptide may be involved in the regulation of testosterone secretion in pubertal male rats.

Full Article: http://zsp.com.pk/pdf/799-803 _24_ PJZ-513-10.pdf
 

Phattony

Member
Registered
Joined
Aug 3, 2013
Messages
557
Effect of obestatin on morphometry of testes and testosterone secretion in male rats
Sarwat Jahan*, Tabinda Sidrat, Shakeel Ahmed, Hizbullah Wazir and Kamran Ullah
Reproductive physiology Laboratory, Department of Animal sciences Quaid-i-Azam University, Islamabad, Pakistan.
Accepted 4 April, 2011
This study was designed to evaluate the effects of chronic intra peritoneal administration of obestatin on plasma testosterone concentrations and cellular morphometry of the testes in male Sprague Dawly rats. The treatment groups were injected with obestatin (1 nmol/100 μl saline i.p), while the control groups received saline (100 μl i.p) for ten consecutive days. Blood samples were collected at day 1 and 10 during the dose administration and day 5 and 15 after the dose administration. All the samples were collected at 10:00 a.m. Testes were removed after sacrificing the rats on days 5 and 15 after the last injection. Plasma testosterone concentrations were found significantly high (p < 0.05) in the obestatin treated groups when compared with the control groups. Testicular histomorphometry revealed that, obestatin treatment caused a significant increase in the primary spermatocytes (P < 0.0001), secondary spermatocytes and spermatids (P < 0.005) and leydig cells population (p < 0.0001) both after 5 and 15 days. These findings indicated that obestatin can be a stimulator of testicular functions.

Full article: http://www.academicjournals.org/ajb/PDF/pdf2011/27July/Jahan et al.pdf
 

Staff online

  • LATS
    Moderator / FOUNDING Member / NPC Judge

Forum statistics

Total page views
502,860,072
Threads
123,552
Messages
2,356,735
Members
155,166
Latest member
Liang
Top