request-step by step mixing igf

[guns123]

could someone please provide me with a link where i can finde step by step procedure for mixing IGF?

responses appreciated

cheers

 

[pesty4077]

could someone please provide me with a link where i can finde step by step procedure for mixing IGF?

responses appreciated

cheers

Just do a search, there is plenty of info on these forums.

 

[guns123]

i have done but there is bits and pieces. i would like to know a step by step thing if possible so i know i am not missing anything/doing something wrong

 

[Builtlilcolt]

Maybe somebody sent you a private message, but just in case their is a reasonable description of "How to Reconstitute" in the article regarding mechano growth factor that someone (sorry) posted here. Look in the forum which has an article about PEG-MGF and the link should be there or go to www.FlawlessTraining.com and, I believe, you can download the article from there.
Reconstituting is pretty staight forward. I imagine it's your final concentraion (mcg per IU) and dosage you are concerned about. It goes over that in the article too. Let me know if that works for you.

 

[guns123]

thankyou, will check it out

 

[Bobaslaw]

could someone please provide me with a link where i can finde step by step procedure for mixing IGF?

responses appreciated

cheers

Here is what you need as posted by REDBARON

Reconstituting IGF - Using Distilled White Vinegar for Acetic Acid

I began using this method many months ago ... mainly out of impatience. I was out of Benzyl Alcohol, and while I had access to HCl, it wasn't accessible when I wanted to begin my Long R3 IGF-1 cycle. I decided to use plain ol' grocery store Distilled White Vinegar. I used the following, which has worked out really well for me....and the feedback I have had from others that I have shared it with has been positive so far. So here it is for anyone that is interested:

The calculation:
Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.

The filtering process:
I use off the shelf grocery store distilled white vinegar. In order to ensure safety, I filter it using .20u whatman filters. Here is the step by step for those that may not be familiar with filtering using whatmans. What you will want to have on hand before starting out is some sterile vials, some .20u whatman filters, some syringes and needles (I use a 10cc syringe, and .23 gauge 1" needles), and some alcohol swabs.

(1) First draw up about 10cc of the distilled white vinegar
(2) screw on the .20u whatman to the 10cc syringe (or whatever size you use)
(3) screw on a .23 gauge needle (or whatever size you decide to use)
(4) take your sterile vial, swab the top with alcohol, insert a needle for venting.
(5) Insert your syringe/whatman/needle apparatus and slowly push the 10cc's into the sterile vial.

Now you have safe vinegar to use for your reconstituting.

ALTERNATE METHOD - Alternately, you could simply mix your water and distilled white vinegar before filtering using about 7.5 parts of water per 1 part of distilled white vinegar. After mixing these together in this ratio, run the mixture through your .20u whatman as above. You will end up with a vial of dilutent this way that has the proper PH for use with your IGF-1.

Reconstituting:

How much water/vinegar you reconstitute with is going to somewhat depend on which LR3 IGF-1 you are using. Igtropin is shipped in 100mcg vials, which I usually reconstitute at 1ml(cc) per 100mcg vial (which will make the 10 mark on your insulin syringe = 10 mcgs). The gropep based IGF-1's are primarily shipped in 1mg vials, and I usually use 5ml for these (which will make the 10 mark on your insulin syringe = 20mcgs).
At any rate, what I do is:

(1) take an alcohol swab and swab the tops of my water, vinegar solution, and IGF-1 vials
(2) take a syringe with a 23 gauge, 1" needle and draw out .12 cc's of vinegar for the 100mcg vials or .60 cc's for the 1mg vials.
(3) next I take this syringe and draw out the water - .88cc's for 100mcg, 4.4cc's for the 1mg.

FOR ALTERNATE METHOD in lieu of steps (2) and (3) - Just draw out the desired amount of dilutent from your pre-mixed vial of
vinegar / water.

(4) next i poke the needle into the LR3 IGF-1 vial and dribble this solution down the side of the vial, avoid any direct spray on the lyophilized powder until all of the dilutent is in the vial
(5) using a gentle swirling motion, I reconstitute the powder.
(6) I stick the vial in the fridge and it is now ready for use.

Well, I think that about sums it up. Hope this helps anyone who may have been wondering about using vinegar to reconstitute. I would advise that if you end up using Igtropin, you seriously consider using this vinegar method. Igtropin and other dilutents such as BA do not get along well together at all.

RedBaron @ IFL

 

[Builtlilcolt]

Diluents ...

Q: Has anyone heard of using bacteriostatic 0.9% sodium chloride as a diluent for any of the peptides (lr3-igf, peg-mgf, cjc-1295)? I have used it to dilute hGH and was under the understanding that it lasts longer in the frig than when sterile water is used (24 hrs). Anybody know?

 

[guns123]

thanks bro, i think i have what i need now. the only other thing is, when mixed with AA (which is what i will be using) i have heard you need to then mix the solution with BW to reduce the injection pain but am not sure on the procedure with this

 

[Bobaslaw]

thanks bro, i think i have what i need now. the only other thing is, when mixed with AA (which is what i will be using) i have heard you need to then mix the solution with BW to reduce the injection pain but am not sure on the procedure with this

It's called backloading. You draw your IGF/AA solution then insert and draw 0.9 % NaCl or BW to dilute. The dilution of the AA solution is not so much to reduce pain (although it does), but in fact it is to minimize the caustic effects of AA on tissue. Keep in mind AA is not something you would normally want to inject as it is very caustic to muscle tissue and on its own in a high enough concentration can cause necrosis of tissue. I usually dilute at a 5:1 ratio.

Take Care.

 

[guns123]

It's called backloading. You draw your IGF/AA solution then insert and draw 0.9 % NaCl or BW to dilute. The dilution of the AA solution is not so much to reduce pain (although it does), but in fact it is to minimize the caustic effects of AA on tissue. Keep in mind AA is not something you would normally want to inject as it is very caustic to muscle tissue and on its own in a high enough concentration can cause necrosis of tissue. I usually dilute at a 5:1 ratio.

Take Care.

coo, so you load syringe as usual..then draw in some BW.obviously i want to keep the total amount of overall liquid low when shooting smaller bp's like bis and tris.so igf injections are painfull huh?
caustic?necrosis?

 

[Bobaslaw]

coo, so you load syringe as usual..then draw in some BW.obviously i want to keep the total amount of overall liquid low when shooting smaller bp's like bis and tris.so igf injections are painfull huh?

If you shoot straight AA you may have some discomfort. I would not call it "painful". BTW, you can shoot a whole cc into your bis and tris just fine, so the actual amount you will use to dilute the AA will be no problem....

caustic?necrosis?

Correct. Acetic Acid is caustic to tissue at a high enough concentration and dosage. For safety it is advisable to dilute the AA solution down.
Don't get me wrong, shooting .6% AA a few times probably won't do significant damage, but pinning that way over time into the same areas will not be good for the local muscle tissue.

 

[Builtlilcolt]

I don't mean to be a pain in the ass, but ...
Based on one of the previous messages, would it be safe to assume that you could use 0.9% NaCl to dilute PEG-MGF and cjc-1295? I just want to make sure I'm not ... what ... denaturing the protein. Would appreciate your help.

 

[Bobaslaw]

I don't mean to be a pain in the ass, but ...
Based on one of the previous messages, would it be safe to assume that you could use 0.9% NaCl to dilute PEG-MGF and cjc-1295? I just want to make sure I'm not ... what ... denaturing the protein. Would appreciate your help.

.9% NaCl is for dilution only. Peptides will not keep well in NaCl. Don not use it to reconstitute peptides. BW is recommended for GHRP/Hex, PegMGF, CJC-1295.

No reason to even contemplate this, just buy some BW online and you will have in a couple days...

 

[66vette]

Diluting AA with what type of water

So you take AA(distilled vinegar), and dilute with what kind of water, distilled water or BA, or sterile water to a 7.5 to1 ratio. Refering to post above. Help please?

Thanks

 

[Bobaslaw]

So you take AA(distilled vinegar), and dilute with what kind of water, distilled water or BA, or sterile water to a 7.5 to1 ratio. Refering to post above. Help please?

Thanks

Distilled Water.

 

[papapumpsd]

I have put together this series of instructions based on board "gurus". I hope this helps some people. Below are the directions I've assembled that I will follow. Adjust for your dosing, syringe type, etc.

I. Making 0.6% acetic acid solution from vinegar:
A) Mix 1mL vinegar with 7.5mL water (1 part vinegar to 7.5 parts water)
B) Attach a 0.20-0.22um sterile Whatman filter to the end of a syringe, then a sterile needle to the end of the Whatman filter
C) Swab the top of a STERILE vial (let dry)
D) Inject the 8.5mL solution into a STERILE vial

II. IGF-1 reconstitution:
A) Swab the top of the IGF-1 vial with an alcohol pad (let dry)
B) Gently introduce 1mL of the 0.6% AA solution made in part I. above. (This yields 1mg/mL solution)
C) Swirl the mixture gently until it is clear and free of visible solid IGF-1

III. Back-loading with BW prior to injection using a 0.5mL slin pin:
A) Swab BOTH tops of the IGF-1 vial and bacteriostatic water vial/bottle with an alcohol pad (let dry)
B) Using a new, sterile 0.5cc slin pin, draw the IGF-1/AA solution up to the 4th tick mark (yielding 40mcg of IGF-1) (I'm dosing 40 mcg bilat, IM)
C) Draw BW to the 20th "tick" mark. This will yield a 4:1 dilution of BW:IGF-1/AA

IV. Injection:
A) swab the injection site with an alcohol pad (let dry)
B) Inject the IGF-1 solution bilaterally (example: 20mcg into right bicep, 20mcg into left bicep)
C) Dispose of syringe in appropriate sharps disposal. Now get your swole on!

GOOD LUCK DOOD!