Thymosin Beta 4(TB-500)

[TestRun]

I did a 3 month cycle of TB500 after shoulder surgery.

Seemed to work, but I'm not sold on the price/results ratio at the moment.

 

[sodzl]

Do you think this would help with bulging discs?

Would like to know too.

 

[johnjuanb1]

Do you think this would help with bulging discs?

Not sure.

 

[Saizen]

If there's a peptide that would help with buldging discs, please let me know.

 

[johnjuanb1]

Thymosin beta4 enhances repair by organizing connective tissue and preventing the appearance of myofibroblasts.

AuthorsEhrlich HP, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:118-24. doi: 10.1111/j.1749-6632.2010.05483.x.

Affiliation
Abstract
Incisional wounds in rats treated locally with thymosin beta4 (Tbeta4) healed with minimal scaring and without loss in wound breaking strength. Treated wounds were significantly narrower in width. Polarized light microscopy treated wounds had superior organized collagen fibers, displaying a red birefringence, which is consistent with mature connective tissue. Control incisions had randomly organized collagen fibers, displaying green birefringence that is consistent with immature connective tissue. Immunohistology treated wounds had few myofibroblasts and fibroblasts with alpha smooth muscle actin (SMA) stained stress fibers. Polyvinyl alcohol sponge implants placed in subcutaneous pockets received either carrier or 100 microg of Tbeta4 on days 2, 3, and 4. On day 14, treated implants revealed longer, thicker collagen fiber bundles with intense yellow-red birefringence by polarized light microscopy. In controls, fine, thin collagen fiber bundles were arranged in random arrays with predominantly green birefringence. Controls contained mostly myofibroblasts, while few myofibroblasts appeared in Tbeta4 treated implants. Electron microscopy confirmed both cell types and the degree of collagen fiber bundle organization. Our results demonstrate that Tbeta4 treated wounds appear to mature earlier and heal with minimal scaring.

 

[johnjuanb1]

Animal studies with thymosin beta, a multifunctional tissue repair and regeneration peptide.

AuthorsPhilp D, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:81-6. doi: 10.1111/j.1749-6632.2010.05479.x.

Affiliation
Abstract
Studies in various animal models of disease and repair with thymosin beta(4) (Tbeta(4)), the major actin-sequestering molecule in mammalian cells, have provided the scientific foundation for the ongoing dermal, corneal, and cardiac wound repair multicenter clinical trials. Tbeta(4) has of multiple biological activities, which include down-regulation of inflammatory chemokines and cytokines, and promotion of cell migration, blood vessel formation, cell survival, and stem cell maturation. All of these activities contribute to the multiple wound healing properties that have been observed in animal studies. This paper reviews and discusses the topical and systemic uses of Tbeta(4) in various animal models that demonstrate its potential for clinical use.

 

[dutchdoc]

TB-500 Definately works

Over a year of experience 2mg per week. ALL of the studies seem to be relevant.

 

[johnjuanb1]

REDUCES INFLAMMATION....

Thymosin beta(4) reduces lethality and down-regulates inflammatory mediators in endotoxin-induced septic shock.

AuthorsBadamchian M, et al. Show all Journal
Int Immunopharmacol. 2003 Aug;3(8):1225-33.

Affiliation
Abstract
Thymosin beta(4) (Tbeta(4)), a highly conserved peptide with immunomodulatory properties, is the major actin-sequestering peptide in mammalian cells. Recent studies have established that Tbeta(4) can accelerate wound healing in full thickness skin wounds and following burn injuries to the cornea. In the eye studies, the accelerated healing due to Tbeta(4) was accompanied by a significant reduction in polymorphonuclear leukocyte (PMN) infiltration and a several-fold decrease in interleukin-1beta (p< or =0.015) and 6-keto-prostaglandin F(1alpha) (6-keto-PGF1alpha, p< or =0.05). Given the recognized role of proinflammatory cytokines in septic shock and of extracellular F- and G-actin in the pathophysiology of multiple organ dysfunction, we have investigated the role of Tbeta(4) in sepsis. We report that an LD(50) dose of LPS (24 mg/kg) in rats resulted in a significant reduction of Tbeta(4) levels in the blood. Furthermore, administration of 100 microg of Tbeta(4) immediately following and at 2 and 4 h after an LD(50) dose of LPS (60 mg/kg) in mice significantly reduced mortality rates (p< or =0.024) and lowered blood levels of a number of inflammatory cytokines, eicosanoids, and other molecules that are highly elevated following endotoxin administration. In studies in human subjects given low doses of endotoxin (4 ng/kg LPS) and in patients with septic shock, we have also observed significant decreases in blood levels of Tbeta(4). The rapid disappearance of Tbeta(4) in the blood following LPS administration or during septic shock suggests that Tbeta(4) may be involved in early events leading to activation of the inflammatory cascade and ultimately the clinical sequelae of sepsis. The results of this study indicate that Tbeta(4) may have utility in the clinic in the treatment of septic shock and in syndromes associated with actin toxicities.

 

[Marky Boy]

I have used tb500 to good effect on my Shoulder injury last year.

Currently looking for more, but it's extremely hard to find a good solid reputable company with high grade peps as this stuff ain't cheap!

 

[johnjuanb1]

I have used tb500 to good effect on my Shoulder injury last year.

Currently looking for more, but it's extremely hard to find a good solid reputable company with high grade peps as this stuff ain't cheap!

Superior has high quality products. Click on my banner. We have a BOGO DEAL going on now.

I just ordered BPC 157 for my minor pec injury. I'll post up results. It'll be the first time I've used BPC 157.

 

[johnjuanb1]

Thymosin beta 4 induces hair growth via stem cell migration and differentiation.

AuthorsPhilp D, et al. Show all Journal
Ann N Y Acad Sci. 2007 Sep;1112:95-103.

Affiliation
Abstract
Thymosin beta 4 is a small 43-amino-acid molecule that has multiple biological activities, including promotion of cell migration angiogenesis, cell survival, protease production, and wound healing. We have found that thymosin beta 4 promotes hair growth in various rat and mice models including a transgenic thymosin beta 4 overexpressing mouse. We have also determined the mechanism by which thymosin beta 4 acts to promote hair growth by examining its effects on follicle stem cell growth, migration, differentiation, and protease production.

 

[johnjuanb1]

Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth.

AuthorsCha HJ, et al. Show all Journal
Int J Dev Biol. 2010;54(1):135-40. doi: 10.1387/ijdb.082735hc.

Affiliation
Abstract
Thymosin beta 4 has multi-functional roles in cell physiology. It accelerates wound healing, hair growth and angiogenesis, and increases laminin-5 expression in corneal epithelium. Furthermore, thymosin beta 4 stimulates tumor growth and metastasis by induction of cell migration and vascular endothelial growth factor-mediated angiogenesis. Using a construct on the skin-specific keratin-5 promoter, we have developed thymosin beta 4 over-expressing transgenic mice to further study its functional roles. Thymosin beta 4 in adult skin and in embryonic stages of the transgenic mouse was analyzed by both Western blot and immunohistochemistry. The over-expression of thymosin beta 4 was observed especially around hair follicles and in the teeth in the transgenic mice. We examined the phenotype of the thymosin beta 4 over-expressing mice. Hair growth was accelerated. In addition, the transgenic mice had abnormally-shaped white teeth and dull incisors. We found that the expression of laminin-5 was up-regulated in the skin of the transgenic mice. We conclude that thymosin beta 4 has an important physiological role in hair growth and in tooth development.

 

[johnjuanb1]

Thymosin beta 4 sulfoxide is an anti-inflammatory agent generated by monocytes in the presence of glucocorticoids.

AuthorsYoung JD, et al. Show all Journal
Nat Med. 1999 Dec;5(12):1424-7.

Affiliation
Abstract
The possibility that glucocorticoids upregulate the expression of anti-inflammatory mediators is an exciting prospect for therapy in inflammatory diseases, because these molecules could give the therapeutic benefits of steroids without toxic side effects. Supernatants from monocytes and macrophages cultured in the presence of glucocorticoids increase the dispersion of neutrophils from a cell pellet in the capillary tube migration assay. This supernatant factor, unlike other neutrophil agonists, promotes dispersive locomotion of neutrophils at uniform concentration, lowers their adhesion to endothelial cells, inhibits their chemotactic response to fMLP and induces distinctive morphological changes. Here we show that thymosin beta4 sulfoxide is generated by monocytes in the presence of glucocorticoids and acts as a signal to inhibit an inflammatory response. In vitro, thymosin beta4 sulfoxide inhibited neutrophil chemotaxis, and in vivo, the oxidized peptide, but not the native form, was a potent inhibitor of carrageenin-induced edema in the mouse paw. Thymosin beta4 is unique, because oxidation attenuates its intracellular G-actin sequestering activity, but greatly enhances its extracellular signaling properties. This description of methionine oxidation conferring extracellular function on a cytosolic protein may have far-reaching implications for future strategies of anti-inflammatory therapy.

 

[Elvia1023]

I modified my leg training I haven't really had anymore knee issues so never bought this. Pretty much everyone I have read about using this as had great results. I am lifting much lighter in general so hopefully that will prevent any bad injuries. Although if I ever injure myself badly I will be jumping on this.

 

[johnjuanb1]

Thymosin β4 enhances the healing of medial collateral ligament injury in rat.

AuthorsXu B, et al. Show all Journal
Regul Pept. 2013 Jun 10;184:1-5. doi: 10.1016/j.regpep.2013.03.026. Epub 2013 Mar 21.

Affiliation
Abstract
The role played by thymosin β4 (Tβ4) in the process of wound healing was reported in several organs. However, there have been no reports that investigated the role of Tβ4 in the repair process after ligament injury. The purpose of this study was to determine whether administration of Tβ4 would improve ligament repair following injury. The medial collateral ligament (MCL) was sharply transected on the day of surgery. Then, the treatment group received 100 μL of fibrin sealant containing 1 μg of Tβ4 placed in the ligament gap. Healing tissues were evaluated by hematoxylin and eosin stain, transmission electron microscopy, and biomechanical test at 4 weeks after surgery. Histologically, healing tissues in Tβ4-treated group exhibited uniform and evenly spaced fiber bundles. However, the collagen fibers were not evenly spaced in control rats. Moreover, diameters of collagen fibrils within granulation tissue from the Tβ4-treated rats were significantly increased. In Tβ4-treated MCLs, the mechanical properties of these healing tissues were significantly higher at 4 weeks after surgery. In terms of the mechanical properties of the healing femur-medial collateral ligament-tibia complexes, the Tβ4-treated group had significantly better biomechanical properties than the control group at 4 weeks after surgery. Local administration of Tβ4 promotes the healing process of MCL, both histologically and mechanically, in a rat model. These findings provide a basis for potential clinical use of Tβ4 in repairing ligaments.

 

[johnjuanb1]

TB500 is a synthetic version of Thymosin Beta-4 which does all the same wonderful things that its natural counterpart does. Some of the claims made by makers of TB500 include:

*Endothelial (blood vessels) cell differentiation
*Angiogenesis (growth of new blood cells from pre-existing vessels) in dermal tissues
*Keratinocyte migration
*Collagen deposition; and
*Decreases inflammation.

 

[johnjuanb1]

A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing.

AuthorsXu TJ, et al. Show all Journal
Drug Des Devel Ther. 2013 Oct 1;7:1075-88. doi: 10.2147/DDDT.S50183.

Affiliation
Abstract
OBJECTIVE: Thymosin beta 4 (Tβ4) is a peptide with 43 amino acids that is critical for repair and remodeling tissues on the skin, eye, heart, and neural system following injury. To fully realize its utility as a treatment for disease caused by injury, the authors constructed a cost-effective novel Tβ4 dimer and demonstrated that it was better able to accelerate tissue repair than native Tβ4.

METHODS: A prokaryotic vector harboring two complete Tβ4 genes with a short linker was constructed and expressed in Escherichia coli. A pilot-scale fermentation (10 L) was performed to produce engineered bacteria and the Tβ4 dimer was purified by one-step hydrophobic interaction chromatography. The activities of the Tβ4 dimer to promote endothelial cell proliferation, migration, and sprouting were assessed by tetramethylbenzidine (methylthiazol tetrazolium), trans-well, scratch, and tube formation assays. The ability to accelerate dermal healing was assessed on rats.

RESULTS: After fermentation, the Tβ4 dimer accounted for about 30% of all the bacteria proteins. The purity of the Tβ4 dimer reached 98% after hydrophobic interaction chromatography purification. An average of 562.4 mg/L Tβ4 dimer was acquired using a 10 L fermenter. In each assay, the dimeric Tβ4 exhibited enhanced activities compared with native Tβ4. Notably, the ability of the dimeric Tβ4 to promote cell migration was almost two times higher than that of Tβ4. The rate of dermal healing in the dimeric Tβ4-treated rats was approximately 1 day faster than with native Tβ4-treated rats.

CONCLUSION: The dimeric Tβ4 exhibited enhanced activity on wound healing than native Tβ4, and the purification process was simple and cost-effective. This data could be of significant benefit for the high pain and morbidity associated with chronic wounds disease. A better strategy to develop Tβ4 as a treatment for other diseases caused by injuries such as heart attack, neurotrophic keratitis, and multiple sclerosis was also described.

 

[johnjuanb1]

Thymosin Beta 4, also known as TB500 or TB-500, is a synthetic version of the naturally occurring Peptide present in virtually all-human and animal cells, Thymosin Beta 4 (T4). It is a first-in-class candidate that promotes the following*:

WHAT THIS DOES:

1. Increases Red Blood Cells

2. Stops bleeding

3. Increase Endurance

4. Reduces Tie Up

5. Helps breathing

6. Reduces stomach acid which eliminates ulcers

7. Increases lean muscle mass

8. Helps repair tendons and ligaments

 

[johnjuanb1]

Thymosin beta4 and cardiac repair.

Shrivastava S, et al. Show all Journal
Ann N Y Acad Sci. 2010 Apr;1194:87-96. doi: 10.1111/j.1749-6632.2010.05468.x.

Affiliation
Abstract
Hypoxic heart disease is a predominant cause of disability and death worldwide. As adult mammals are incapable of cardiac repair after infarction, the discovery of effective methods to achieve myocardial and vascular regeneration is crucial. Efforts to use stem cells to repopulate damaged tissue are currently limited by technical considerations and restricted cell potential. We discovered that the small, secreted peptide thymosin beta4 (Tbeta4) could be sufficiently used to inhibit myocardial cell death, stimulate vessel growth, and activate endogenous cardiac progenitors by reminding the adult heart on its embryonic program in vivo. The initiation of epicardial thickening accompanied by increase of myocardial and epicardial progenitors with or without infarction indicate that the reactivation process is independent of injury. Our results demonstrate Tbeta4 to be the first known molecule able to initiate simultaneous myocardial and vascular regeneration after systemic administration in vivo. Given our findings, the utility of Tbeta4 to heal cardiac injury may hold promise and warrant further investigation.

 

[johnjuanb1]

One of Thymosin Beta 4's key mechanisms of action is its ability to regulate the cell-building protein, Actin, a vital component of cell structure and movement. Of the thousands of proteins present in cells, actin represents up to 10% of the total proteins which therefore plays a major role in the genetic makeup of the cell.