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1. GHRH CJC 1295 w/DAC causes prolonged secretion of GH from the pituitary. In normal physiology, once GH has initiated the secretion of IGF-1 (somatomedins), which inhibit further secretion of GH from the pituitary and by stimulating the secretion of somatostatin from the hypothalamus. GHRH also inhibits its own secretion from the hypothalamus. GH secretion also causes secretion of somatostatin (directly inhibits GH) from the hypothalamus thus inhibiting its own secretion as well. These negative feedback loops would lead me to believe the use of CJC 1295 w/DAC would not be as effective as GHRH w/out DAC.
2. Having said that, there is new data to show the potential usefulness of the peptide CJC1295 DAC, recent studies have shown a 7.5 fold increase in basel GH levels and a 1-3fold increase in IGF-1 levels when using doses of 30-60mcg per kg, so for a 100kg person this would be 3-6mg per week. These studies were not compared to the use of GHRH w/out DAC:
J Clin Endocrinol Metab. 2006 Mar;91(3):799-805. Epub 2005 Dec 13.
J Clin Endocrinol Metab. 2006 Dec;91(12):4792-7. Epub 2006 Oct 3.
Pulsatile GH secretion is considered important for many of the hormone’s physiological effects. Short-term GHRH infusions enhance GH pulsatility and increase IGF-I, but the short GHRH half-life limits its therapeutic use. A synthetic GHRH analog (CJC-1295) that binds permanently to endogenous albumin after injection (half-life = 8 d) stimulates GH and IGF-I secretion in several animal species and in normal human subjects and enhances growth in rats. GH secretion was increased after CJC-1295 administration with preserved pulsatility. The frequency and magnitude of GH secretory pulses were unaltered. However, basal GH levels were markedly increased (7.5-fold; P < 0.0001) and contributed to an overall increase in GH secretion (mean GH levels, 46%; P < 0.01) and IGF-I levels (45%; P < 0.001). The marked enhancement of trough GH levels by continuous GHRH stimulation implicates the importance of this effect on increasing IGF-I. Long-acting GHRH preparations may have clinical utility in patients with intact pituitary GH secretory capability.
3. GH works in a fashion that insulin hence any fat or carbohydrates will block or inhibits its secretion. Therefore, it is very important when trying to initiate GH secretion, even with the use of peptides like GHRP or GHRH that you be in a fasted state. You may eat pure protein but as a rule of thumb it is good to remain completely fasted for maximal GH secretion. A good period of fasting would be 2 hours previous to any injection. 2 hours is approximately how long it take for the stomach to empty its contents into the duodenum. Once you have inject, it is best to stay fasted for a complete 30 min. Some say 15-20 but for maximal GH secretion try to make it to 30 min (may be very hard with GHRP6 lol).
4. BPC 157 Peptide is a new great healing peptide. It appears that it cause new vessel formation at injection site and has even proven to assist in the healing of a rat’s quadriceps. There is suggested use of 250-500mcg daily split between each injured tendon area. Some have taken upwards to 800mcg a day but as little as 200mcg has shown to be effective. Broken down in to 2x/day injections may be more beneficial. Injecting right into the tendon or joint is very dangerous and that must be taken in to consideration. So if you choose, do it at your own risk. Injection site, preferably around 1-4 inches away to yield the most benefit to the injured area. In summation, both healing Peptides are capable of healing injuries systemically or locally via subcutaneous or intramuscular injection.
Some have found intramuscular injection into the wound site to be best, requiring only minimal doses. It appears BPC157 works by stimulating angiogenisis (creation of new blood vessels) which in turn aids in healing. Frequent micro-dosing as close as possible to the injury site as possible seems to be good. After all you cannot create any slow release deposit of a water soluble as you do with oils; also, keep in mind at the cellular level you only need that very small quantity to hit the receptors or trigger whatever events lead to the healing. Therefore, I argue that small frequent administrations will lead to a greater overall exposure at the site you wish to stimulate the angiogenisis (and resulting increased healing) upon as oppose to one larger less frequent dosage that simply gets swept away. Some say they healed within 10 days with this protocol.
“The beneficial effect of BPC 157, a 15 amino acid peptide BPC fragment, on gastric and duodenal lesions induced by restraint stress, cysteamine and 96% ethanol in rats. A comparative study with H2 receptor antagonists, dopamine promotors and gut peptides”
Predrag Sikiric MD PhD, CDD, Department of Pharmacology, Medical Faculty University of Zagreb, Salata 11, POB 916, 41000 Zagreb, Croatia
European Journal of Pharmacology
“The influence of a novel pentadecapeptide, BPC 157, on NG-nitro-L-arginine methylester and L-arginine effects on stomach mucosa integrity and blood pressure”
Journal of Orthopedic Research
“Effective therapy of transected quadriceps muscle in rat: Gastric pentadecapeptide BPC 157”
5. Adding 1-4iu of GH 10-15 minutes after peptides will give a bigger overall pulse of GH (natural + synthetic) any more than 4iu and this will result in more of a bleed type situation rather than a pulse of GH.
Adding Insulin to peptides will give you the same type of results as adding it to GH
6. You touched on sleep. There is a peptide that is actually really remarkable called DSIP or Delta Sleep Inducing Peptide. I don't have time to discuss it now but this would be an amazing discussing as I have even read that this peptide can extend life and reduce the risk of tumors (cancer). Which may come to be important if take excess IGF-1. There is also another peptide worth discussing called Epithalon, which I believe has had some twin studies done in Russia I believe. Crazy results.
