- Joined
- Mar 1, 2013
- Messages
- 3,728
Anyone see QSC GH test results where purity came back at 100% but just slightly off form of pharma HGH ?
This may explain differences effects experienced between generics and pharm.
"Attached are SDS-PAGE gels of the most recent lot of growth hormone that was sent to me. Purity came out to be 100% at 2 ug. Industry standard usually observes silver g stain gels at 1ug, so the purity is impressive. The only thing that concerned me was the band shows at ~20 kDa. Human growth hormone is published to be 22kDa. After researching Uniprot, there are 5 published isoforms of human growth hormone. This is most likely isoform P01241-2. This means that aa58-72 are differences from canonical. This can be missense variant consequential from experimental artifact such as PCR, cloning, or reverse transcripts errors. Regardless, I have been using the product myself for three months and it is as advertised, isoform or not, but it is not the same quality as pharma based on SDS-PAGE. For 1/10 the price I would not expect it to be either."
This may explain differences effects experienced between generics and pharm.
"Attached are SDS-PAGE gels of the most recent lot of growth hormone that was sent to me. Purity came out to be 100% at 2 ug. Industry standard usually observes silver g stain gels at 1ug, so the purity is impressive. The only thing that concerned me was the band shows at ~20 kDa. Human growth hormone is published to be 22kDa. After researching Uniprot, there are 5 published isoforms of human growth hormone. This is most likely isoform P01241-2. This means that aa58-72 are differences from canonical. This can be missense variant consequential from experimental artifact such as PCR, cloning, or reverse transcripts errors. Regardless, I have been using the product myself for three months and it is as advertised, isoform or not, but it is not the same quality as pharma based on SDS-PAGE. For 1/10 the price I would not expect it to be either."