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Acetic Acid 0,6% - How much ml in vial of Igf-1 Long3 1ml?

kkmark81

Banned
Joined
May 16, 2007
Messages
23
Hi Bro,

How much ml must in one vial of generic igf-1 long3 (1mg) for being stable?

I have this vial Sterile Acetic Acid 100mM 25ml (Acetic Acid 0.6%), it goes well in order to reconstitute the product?

How much it remains stable?

To that temperature, can be the reconstituted product?

Thanks to all.
 
IGF is stable for a long time in 0.6% acetic acid when stored in the refrigerator.

This is direct info from CHEMONE:

mixing directions

first remove the vacuum from the vial, by injecting air into it.
when the vacuum is gone it will no longer pull the plunger down

you can use as much AA as you like. the original kits came with
1ml. most time when i drew it into a syringe, it was't even that
much, which of course is a problem. chemone includes a little more
than 2ml so if you want to use 1ml, fine, 2ml, fine. there will be
enough.

i personally use 2ml because it is easier to measure later when you
draw it into a syring.

after removing the vacuum SLOWLY add AA to the vial. try not to let
the stream of aa hit the powder directly, try to let it run down
the glass into the powder. when the aa is in gently swirl the vial
until all is disolved. do not shake.

a 1ml syringe is marked like this
llll10llll20llll30llll40llll50llll60...etc
a 1/2 ml syringe is like this llll5llll10llll15llll20llll25llll30

on a 1ml each line represents 2 units 2,4,6,8,10
on a 1/2 ml each line is 1 unit 1,2,3,4,5,6,7,8,9,10. this makes it
easier to measure.

so if you use 1ml of aa, on a 1ml pin, each line is 20mcg. on a
1/2ml pin each line is 10mcg

if you use 2ml of aa, on a 1ml pin each line is 10mcg, on a 1/2 ml
each line is 5mcg...the 5line is 25mcg, 10 line is 50mcg...etc

so you see that the easiest way to measure is using 2ml and a 1/2ml
pin

your igf is stored in aa solution, nacl is added to dilute at the
time of injection. draw twice as much nacl as igf. so if you draw
igf to the ten line, draw nacl to the 30 line and you are ready to
go.

chemone's kits are exactly how they should be. a true .6% aa
solution and nacl(saline solution) to dilute. diluting lessens the
sting from the acid and prevents tissue necrosis.
 
Thanks to all....this protocol is good for vials of 0,1mg and 1mg?
 
Last edited:

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