Here is a good protocol to help you have some fun with your new toy.
IGF1
PROTOCOL
UNOFFICIAL IGF1
INSTRUCTIONS
(1) Storage of IGF1
(prior to mixing)
Lyophilized (dry) IGF1
is stable at room temperature for 3 weeks; however, it
should be stored below 18
degrees Celsius (in the freezer section).
(2) Weight of IGF1
1 milligram (mg) IGF1
= 1000 micrograms (mcg) IGF1
[Dry weight—before mixing]
(3) What to Mix the IGF1
with
When reconstituting (adding water), It is important to remember that IGF1
can
get “stuck” in the grooves of the glass bottle it comes packaged in. While glass appears
smooth to the naked eye, under a microscope, it is a convoluted landscape of grooves and
hidden recesses.
By mixing the lyophilized IGF1
with an “acid water” (e.g. 10mM HCLvery
dilute hydrochloric acid), the IGF1
molecules are efficiently detached from the glass and
solubilized in the mixture. Any online “compounding” laboratory could mix up a 10mM
HCL solution. Likewise, any intro chemistry student should be able to do the same.
If a reliable source of “acid water” cannot be located, mix your IGF1
powder
with bacteriostatic water—you’ll lose, at worst, 10% of the IGF1
solution.
(4) Adding the Acid Water
For purposes of mathematical ease, I suggest mixing the dry 1mg (1000mcg) IGF1
with 3mL (or 3cc) of the “acid water” mixture.
(5) Preservation of the IGF1
Using a 1cc insulin syringe, draw 1cc out of the bottle that contains 3cc of the
acid water/IGF1
mixture. In a separate 1cc insulin syringe, draw up another 1cc of the
solution. Freeze these two loaded insulin syringes. They will be utilized at a later date.
NOTE: Freezing can safely and effectively preserve IGF1
(even after it’s been mixed).
(6) The Correct Dilution
To the remaining 1cc of IGF1
that’s left in the glass bottle, add 2cc of
bacteriostatic water. This will return the total volume back up to 3cc.
(7) The Mathematics
(a) The original concentration of the IGF1
solution was 1mg (1000mcg) IGF1
in
3cc of water.
(b) Each 1cc that we removed, then, contained approximately 333mcg per 1cc.
1000mcg / 3cc = 333mcg per 1cc
(c) The 1cc that was left in the bottle, then, also contains 333mcg of IGF1.
(d) We added 2cc of bacteriostatic water to the bottle and brought the total
volume back up to 3cc. The difference, now, is that we now have 333mcg in 3cc of
liquid (instead of in 1cc)
(e) To determine how much IGF1
is in 1cc, you must divide by 3
333mcg / 3cc = 111mcg per 1cc
(f) To determine how much IGF1
is in .10cc (or 1/10 th cc), we do the following:
111mcg / 10 = 11mcg per .10cc (or 10 unit marking on insulin syringe)
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