Heat Sterilization

[mgangers]

Hi Guys,

Maybe a dumb question but I will ask anyway, is is possible to burn the oil while sterilizing Test e, the reason I ask is I baked my Test e (T300) and the oil came out darker then it went in.

Thanks for the help.

 

[mgangers]

follow up to last post

just realized that my last post was very vague

sorry for being vague, here is the clarification.

baked test e at 350 degrees for 60 minutes, then inserted an 18ga pin in rubber stopper to vent vial, gear was almost clear going into the oven but almost golden when it came out of the oven.

Again, thanks for the help

 

[Quad-smack]

Pretty sure you destroyed it at 350 degrees. Autoclave sterilization is done at 250 degrees for 30 minutes.

 

[Bigblack]

No need to use heat to sterilize, the whatman filter will do that. You probably caused some oxidation in the TE by heating it to such a high degree. The heat should merely be used to facilitate faster "mixing".

 

[stinkpelt]

No need to use heat to sterilize, the whatman filter will do that. You probably caused some oxidation in the TE by heating it to such a high degree. The heat should merely be used to facilitate faster "mixing".

So.....using a whatman filter will totally sterilize gear? Is this something that should be done with every vial just to be on caution's side? I have wondeed this for a while. I usually just pin what is in the vial but I sometimes worry that maybe I should sterilize just to be on the safe side.

Suggestions....

 

[Bigblack]

So.....using a whatman filter will totally sterilize gear? Is this something that should be done with every vial just to be on caution's side? I have wondeed this for a while. I usually just pin what is in the vial but I sometimes worry that maybe I should sterilize just to be on the safe side.

Suggestions....

That would depend on your source. Trust your source-no need to filter. I should also note, for the sake of being thorough, that the BA will, in part, kill some gram (-) bacteria, due to dehydration of the LipidPolysaccharide layer.

I believe the original poster was talking about making his own- in which case filtering through a .22um whatman filter is a MUST.

 

[Stewie Griffon]

agree with using a whatman filter, its pore size is so small it physically filters out bacteria - they are too large to pass through; like grains of sand are too large to pass through a coffee filter.

heat will kill bacteria, but think, what happens to them? they dont disappear they are just dead, so any bacteria that were present and killed by heat are now little dead carcasses. filtering is the way to go

 

[Massive G]

Most people missed the point of my original baking post.
The purpose is to reduce the pain from the solvent and offer more security after initial (clarification) filtering with .45 and .2 um filters even sterile do not remove ALL the bacteria-there are endotoxins as well as mycoplasma will get through a .2 um filter.

You didn't destroy your gear, the temp should be around 300 for 30 minutes. the oil will darken with heat and time, but the hormone itself is not "poof" ala degraded.

Tens of thousands of people over the years have been 'baking" gear to sterilize with and without filtration for over a decade plus (RE: Fina kits and IP's gear) and they grew like weeds off it and were pain free.

Anyone that takes a bottle of gear obtained off the black market and pops the tops and injects it is taking a risk of sterile abcess from the high solvent level or a bacterial infection.

for the most part gear isn't heavily contaminated but the residues in it and solvent can cause more problems than a guy needs and make him miserable with test flu.

So much information on these boards is spread due to inexperience, most of those fears have been proven wrong by thousands of test cases over the years if not more.

Don't even get me started on peptides that's a whole new realm of (idiocy) -inexperience.

I don't like to call people dumb they just don't know any better is all.

 

[renewlyf]

Does Baking it Ease the Pain?

I'm currently using some stealth products. It doesn't hurt immediately but does ball up in the glute and is sore for a couple of days. Will heating it help?

 

[EasyEJL]

I'm currently using some stealth products. It doesn't hurt immediately but does ball up in the glute and is sore for a couple of days. Will heating it help?

it should. particularly if there isn't any pain the injection day and it develops the following day, thats generally a sign of your body fighting infection. If the pain is due to high solvent content, it should start hurting within minutes to maybe 1/2 hr after injecting

 

[Bigblack]

Most people missed the point of my original baking post.
there are endotoxins as well as mycoplasma will get through a .2 um filter.

.

Endotoxins are so rare-if the given person is baking in a clean environment. How many people have Ecoli O157 or shigella roaming around on their clean kitchen countertop? Yes-all mollicutes and virons would fit thorugh a whatman filter, but unless you have access to an FDA certified clean room, whatman filter is the safest way to go. Baking your gear-dry heat sterilization would be generally ineffective on microorganisms that lack a cell wall anyway.

 

[mgangers]

follow-up

Just thought I would provide an update

started my cycle with gear I got from one of the sponsor, first shot in the right quad, had pain for 6 days, limping around like a gimp pain!

Baked the gear, took my second shot, no pain during injection, felt some mild disconfort the next day, other then that nothing.

from now on all my gear is going in the oven before my cycle starts.

 

[The Booger]

Those who filter and bake, are you baking first and then filtering? Or vice-versa?

I ask because I have some old gear (about 2 years old, 5mL out of 5mL used), so I wanted to take the extra steps to make sure it is as sterile as I can get it.

Did a test injection about a week ago and nothing, but that doesn't mean it WON'T happen.

 

[Eureka]

Endotoxins are so rare-if the given person is baking in a clean environment. How many people have Ecoli O157 or shigella roaming around on their clean kitchen countertop? Yes-all mollicutes and virons would fit thorugh a whatman filter, but unless you have access to an FDA certified clean room, whatman filter is the safest way to go. Baking your gear-dry heat sterilization would be generally ineffective on microorganisms that lack a cell wall anyway.

Agreed, the BA is what kills the bacteria also not the heating. The heating is just an added precaution..