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see sticky for aestheticlabs..I'm in a constant state of learning ;what I found true yesterday ,may not hold true today
and there is always a minuscule possibility I'm wrong ....
It may be hard to believe but I did not write the folllowing
Peg hgh seems superior in muscle growth than daily injecions of SYNTROPIN™, NUTROPIN®, and HUMATROPE®
The activity of the preparation described above was tested by the cell proliferation assay with BaF3 cells and compared to commercially available hGHs. SYNTROPIN™, NUTROPIN®, and HUMATROPE® are commercially available hGH forms. A representative standard curve is shown in Figure 9 where similar dose response curves are seen with four commercially available hGHs. The specific activity of hGH prepared as described above compares favorably with commercially available hGH. As expected, pegylation of the native SYNTROPIN™ resulted in a loss of biological activity, with a greater activity loss occurring as one attaches more PEG groups to the native hGH.
Table 1 : Potencies (IU/mg) of hGH Preparations Tested in the Cell Proliferation Assay
Example 6. Effect of PEG-hGH on body growth in male hvpophvsectomized rats
A second bioassay utilized was the classical rat weight gain assay (Roswall EC, et al. 1996. Biologicals 24: 25-39) where the weight of 4-5 week old hypophysectomized rats (Orient, Inc. 143-1 Sangdaewon-dong, Sung-Nam, Kyunggi-do) were monitored over a 28 day period, following subcutaneous hGH injections, once daily, for the first 7 days, or one injection at day 1 of the mono- or di-PEG-S YNTROPIN. Remarkably, and unexpectedly given the rather low potency of the PEG-SYNTROPINS in the cell-based bioassay (Table 1) , the pegylated S YNTROPINS showed equivalent activity with native hGH when injected only once a week versus daily injections for native hGH (see Figure 10). It can be seen in Figure 10 that at 7 days, rats given daily injections of native hGH weighed
approximately the same as rats given a single injection of either the mono-PEG- SYNTROPIN or the di-PEG-SYNTROPIN. Also unexpected was the rate of weight gain in the animals administered PEG-SYNTROPINS versus those animals receiving native SYNTROPIN. hi Figure 10 it can be seen that the rats given a single dose of either mono- PEG-hGH or di-PEG-hGH gained weight at a significantly faster rate over the first 4 days than those animals receiving a daily injection of native hGH. It was not until day 7 that the animals receiving native hGH were able to "catch up" in body weight with the animals receiving the pegylated growth hormones.
The effect of PEG-hGH on body growth in male hypophysectomized rats was further investigated with different doses. The 4-5 week-old hypophysectomized rats were purchased, stored for 5 days, administered PEG-hGH(G6~G8) by s.c. once, and determined the body weights daily for 11 days. A saline solution as a negative control (Vehicle control, Gl) and native hGH (Positive control, G2-G4) were administered by s.c. every day for 6 days (Day 1 - Day 6) and the body weight of each rat was measured for 11 days. Also, a high dose (180 μg) hGH(G5) was administered by s.c. once and the body weight was measured to compare with other groups.
No unusual symptoms were observed as a result of any of the treatments and no significant change in body weight was observed in the saline control (Gl, Figure 1 IA-C) or the hGH administered group (G5, Figure 1 IA, HC). From this study, it was found that the body weights of rats for the positive control,
G2, G3 and G4, were increased by 7.37 %, 9.03 %, and 10.79 %, respectively, during the daily administration of hGH (Figure 1 IA, 1 IB), while the body weights of rats for the PEG- hGH rats, G6, G7 and G8, were increased by 8.42 %, 12.48 %, and 18.37 %, respectively, after single administration of PEG-hGH (Figure HA, 11C). This result shows that the increase of body weight is proportional to the amount of hGH or PEG-hGH administered. However, one bolus administration of high dose of hGH (180ug/rat) produced only a small and transitory response (G5, Figure HA, 11C), whereas a single administration of PEG- hGH gave a much larger and more sustained response.
It was observed that the single injection of PEG-hGH to hypophysectomized rats enhanced the body weight notably and fairly maintained the increased body weight at least for 6 days. The daily injection of hGH enhanced the body weight but the body weight began to decrease as soon as the administration of hGH was stopped. It was also observed that the
single bolus high dose injection of hGH (G5) enhanced body weight by <4 % the day after administration followed by decreased body weight to as low as negative control indicating that the continuous injection of native hGH was necessary to enhance body weight continuously for the non-pegylated hGH samples. Dose dependent body weight gain was observed for hGH as well as PEG-hGH. In other words, the body weight gain was proportional to the amount of hGH or PEG-hGH administered in hypophysectomized rats.
This study shows that single injection of PEG-hGH enhanced body growth and fairly maintained body weight at least for 6 days in hypophysectomized rats whereas daily injection of non-pegylated hGH for 6 days was necessary to maintain the enhanced body weight of rats continuously. PEG-hGH as a weekly injectable drug is a promising alternative to daily injections of hGH.
Thus, while there have been described the preferred embodiments of the present invention, those skilled in the art will realize that other embodiments can be made without departing from the spirit of the invention, which includes all such further modifications and changes as come within the meaning, true scope of the claims set forth herein and equivalents thereof. The above examples further describe and demonstrate embodiments within the scope of the present invention. The examples are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
The cost of peg hgh is @ 700 a month (10mg x2),compared to gh @720 a month(6iux30=180iu x $4).
Depending on How many iui of gh you take a day,which is all relitive to your goals or what one is trying to achieve....
**Its my understandind that peg hgh is not controlled substance,does anyone know something to the contrary?***
love & respect DLD
and there is always a minuscule possibility I'm wrong ....
It may be hard to believe but I did not write the folllowing
Peg hgh seems superior in muscle growth than daily injecions of SYNTROPIN™, NUTROPIN®, and HUMATROPE®
The activity of the preparation described above was tested by the cell proliferation assay with BaF3 cells and compared to commercially available hGHs. SYNTROPIN™, NUTROPIN®, and HUMATROPE® are commercially available hGH forms. A representative standard curve is shown in Figure 9 where similar dose response curves are seen with four commercially available hGHs. The specific activity of hGH prepared as described above compares favorably with commercially available hGH. As expected, pegylation of the native SYNTROPIN™ resulted in a loss of biological activity, with a greater activity loss occurring as one attaches more PEG groups to the native hGH.
Table 1 : Potencies (IU/mg) of hGH Preparations Tested in the Cell Proliferation Assay
Example 6. Effect of PEG-hGH on body growth in male hvpophvsectomized rats
A second bioassay utilized was the classical rat weight gain assay (Roswall EC, et al. 1996. Biologicals 24: 25-39) where the weight of 4-5 week old hypophysectomized rats (Orient, Inc. 143-1 Sangdaewon-dong, Sung-Nam, Kyunggi-do) were monitored over a 28 day period, following subcutaneous hGH injections, once daily, for the first 7 days, or one injection at day 1 of the mono- or di-PEG-S YNTROPIN. Remarkably, and unexpectedly given the rather low potency of the PEG-SYNTROPINS in the cell-based bioassay (Table 1) , the pegylated S YNTROPINS showed equivalent activity with native hGH when injected only once a week versus daily injections for native hGH (see Figure 10). It can be seen in Figure 10 that at 7 days, rats given daily injections of native hGH weighed
approximately the same as rats given a single injection of either the mono-PEG- SYNTROPIN or the di-PEG-SYNTROPIN. Also unexpected was the rate of weight gain in the animals administered PEG-SYNTROPINS versus those animals receiving native SYNTROPIN. hi Figure 10 it can be seen that the rats given a single dose of either mono- PEG-hGH or di-PEG-hGH gained weight at a significantly faster rate over the first 4 days than those animals receiving a daily injection of native hGH. It was not until day 7 that the animals receiving native hGH were able to "catch up" in body weight with the animals receiving the pegylated growth hormones.
The effect of PEG-hGH on body growth in male hypophysectomized rats was further investigated with different doses. The 4-5 week-old hypophysectomized rats were purchased, stored for 5 days, administered PEG-hGH(G6~G8) by s.c. once, and determined the body weights daily for 11 days. A saline solution as a negative control (Vehicle control, Gl) and native hGH (Positive control, G2-G4) were administered by s.c. every day for 6 days (Day 1 - Day 6) and the body weight of each rat was measured for 11 days. Also, a high dose (180 μg) hGH(G5) was administered by s.c. once and the body weight was measured to compare with other groups.
No unusual symptoms were observed as a result of any of the treatments and no significant change in body weight was observed in the saline control (Gl, Figure 1 IA-C) or the hGH administered group (G5, Figure 1 IA, HC). From this study, it was found that the body weights of rats for the positive control,
G2, G3 and G4, were increased by 7.37 %, 9.03 %, and 10.79 %, respectively, during the daily administration of hGH (Figure 1 IA, 1 IB), while the body weights of rats for the PEG- hGH rats, G6, G7 and G8, were increased by 8.42 %, 12.48 %, and 18.37 %, respectively, after single administration of PEG-hGH (Figure HA, 11C). This result shows that the increase of body weight is proportional to the amount of hGH or PEG-hGH administered. However, one bolus administration of high dose of hGH (180ug/rat) produced only a small and transitory response (G5, Figure HA, 11C), whereas a single administration of PEG- hGH gave a much larger and more sustained response.
It was observed that the single injection of PEG-hGH to hypophysectomized rats enhanced the body weight notably and fairly maintained the increased body weight at least for 6 days. The daily injection of hGH enhanced the body weight but the body weight began to decrease as soon as the administration of hGH was stopped. It was also observed that the
single bolus high dose injection of hGH (G5) enhanced body weight by <4 % the day after administration followed by decreased body weight to as low as negative control indicating that the continuous injection of native hGH was necessary to enhance body weight continuously for the non-pegylated hGH samples. Dose dependent body weight gain was observed for hGH as well as PEG-hGH. In other words, the body weight gain was proportional to the amount of hGH or PEG-hGH administered in hypophysectomized rats.
This study shows that single injection of PEG-hGH enhanced body growth and fairly maintained body weight at least for 6 days in hypophysectomized rats whereas daily injection of non-pegylated hGH for 6 days was necessary to maintain the enhanced body weight of rats continuously. PEG-hGH as a weekly injectable drug is a promising alternative to daily injections of hGH.
Thus, while there have been described the preferred embodiments of the present invention, those skilled in the art will realize that other embodiments can be made without departing from the spirit of the invention, which includes all such further modifications and changes as come within the meaning, true scope of the claims set forth herein and equivalents thereof. The above examples further describe and demonstrate embodiments within the scope of the present invention. The examples are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
The cost of peg hgh is @ 700 a month (10mg x2),compared to gh @720 a month(6iux30=180iu x $4).
Depending on How many iui of gh you take a day,which is all relitive to your goals or what one is trying to achieve....
**Its my understandind that peg hgh is not controlled substance,does anyone know something to the contrary?***
love & respect DLD
