Juice Freak; said:
Yes it does make sense.
Juice Freak; said:
...and also I would like to incorporate gh frag into the mix at a dose of 1mg x 2 daily. When would be the best time for this and will this increase my igf even though it's supposed not to? Thank you for you help.
Synthetic GH is the naturally occurring isoform known as 22kda (22 kilo daltons of weight) with a 191 amino acid chain structure and really cool 3 dimensional structure.
It binds to a GH receptor which can be visualized as two posts or hold your hand up and make a "V" sign with two of your fingers. The GH peptide contains two sites which will always bind to two specific sites on the receptor (one on each post or one on each finger). GH binds first to one post of the receptor and then the other.
GH has a specific 3 dimensional shape and when it binds to the receptor it twists those two posts. So take your "V" shaped finger and now move one forward. You'll see your hand twist.
When GH binds to the part of the receptor that sticks out from the cell surface (like 2 blades of grass) it twists the receptor and this twist continues down inside the cell. Inside the cell this twists brings certain molecules which previously were not in contact, into contact. This starts a process of which activates several well defined signaling pathways...
...these signaling pathways such as stat5b translocate (or move) to the cell nucleus and effects protein transcription. Acid labile Subunit, IGFBP-3 & IGF-1 eventually end up being newly synthesized in this process.
The body makes several forms of GH. The two anabolic forms are the 191 amino acid 22kda variety and the 20kda variety as well as double stacks contain these two peptides. In addition some fragments may also be released while some parts of the GH chain are released and then cleaved by enzymes to create naturally occurring fragments.
GH1–43 fragment (which is simply the first 43 amino acids of the 191 amino acid full GH peptide) possesses insulin-like activity, but does not appear to have growth-promoting activity.
A subset of GH peptide fragments including GH1–20, GH1–15, GH4–15, GH6–13 have been demonstrated to induce hypoglycaemia in vivo and amplify the actions of insulin in vitro.
GH44 – 191 fragment has neither growth-promoting nor insulin-like activity. It does possess a potent diabetogenic effect (i.e. creating insulin resistance in tissue) with a potency 10-fold higher than the full GH1–191. This fragment binds to one of the GH receptor posts but not the other one and so could act as a GH receptor antagonist (because it blocks the receptor & produces no anabolic effect (i.e. does not start the signaling cascade)).
It does bind to the lactogenic receptors.
GH108 – 129 fragment appears to possess mitogenic activity although intact GH demonstrates anti-mitogenic activity. In other words it commences cell division.
GH177 – 191 is the part of the GH peptide that is responsible for GH's lipolytic activity (i.e. breakdown of fat stored in fat cell). This GH fragment prevents angiogenesis in growing capillaries (physiological process involving the growth of new blood vessels from pre-existing vessels). This is an an opposite effect from the full length GH 1-191 which promotes angiogenesis.
These effects, the lipolytic and the anti-angiogenesis are not mediated through the GH receptor. Instead in the case of lypolysis it has a direct action on adipose tissue. The lipolytic effect is mediated by an increase in hormone-sensitive lipase (HSL) activity. The anti-lipogenic effect (i.e. stopping the forming fat) is created by significantly reducing acetyl-CoA carboxylase activity. This effect is found to be dose dependent.
The GH177 – 191 molecule is stable in aqueous buffered solution with a half-life of 50 to 170 minutes.
GH177-191 has not been demonstrated to occur naturally. It is a man made cleavage but it is the metabolic portion of GH that is responsible for lipolytic/anti-lipogenic activity.
Since it doesn't bind to the GH receptor but instead mediates its actions directly in adipose tissue it will not increase IGF-1 or have any anabolic (anti-catabolic action).
You would dose this anytime you want to increase fat mobilization. In the presence of insulin it is likely to still engage lipolysis because the full GH peptide does, however the maximum lipolytic activity will come when insulin is not present.
Keep in mind that fat mobilization is not sufficient. You still do need some level of base activity to "burn off" mobilized fat.
Okay you can put your hand down now.