I really need your help, Gents! I'd like your opinions on whether I should toss some of my gear. Damned sad times over the pond!
I have 2 questions: After transfering to vials, should I/can I bake the gear to make sure it's sterile? Does this even help? My transfer technique was good, but not perfect IMO. Too much air exposure. I usually use HG so first time with transferring.
Second: I have gear left in the jug. Toss it? I explain how this happened below. Idiocy...and being over-careful. I appreciate your advice, Guys.
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So after sterilizing everything in a mile radius, I start transfering my gear from a jug to sterile vials. It's going ok. Im taking the vials out of a bag to limit any exposure, cleaning them with alcohol, then drawing from the jug and injecting into the bottle.
I had to open the jug top, so there's air exposure. Not good, but not really avoidable. It's taking too long!
Last bottle, I take from the bag and realize to my horror, that it has a hairline crack that it must have got in the mail. The sealed top is also banged up. It's no good, unusable.
So now, I've got about 20ML in the jug still, that's been exposed to air and nowhere to go. Bummer!
If I transfer it to a sterile vial later, and bake it, will this help? Or am I just fooked on these 20ml.
I have 2 questions: After transfering to vials, should I/can I bake the gear to make sure it's sterile? Does this even help? My transfer technique was good, but not perfect IMO. Too much air exposure. I usually use HG so first time with transferring.
Second: I have gear left in the jug. Toss it? I explain how this happened below. Idiocy...and being over-careful. I appreciate your advice, Guys.
----
So after sterilizing everything in a mile radius, I start transfering my gear from a jug to sterile vials. It's going ok. Im taking the vials out of a bag to limit any exposure, cleaning them with alcohol, then drawing from the jug and injecting into the bottle.
I had to open the jug top, so there's air exposure. Not good, but not really avoidable. It's taking too long!
Last bottle, I take from the bag and realize to my horror, that it has a hairline crack that it must have got in the mail. The sealed top is also banged up. It's no good, unusable.
So now, I've got about 20ML in the jug still, that's been exposed to air and nowhere to go. Bummer!
If I transfer it to a sterile vial later, and bake it, will this help? Or am I just fooked on these 20ml.